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High Resolution Flow Cytometry Specialist - Immunology

Department: Flow Cytometry

High Resolution Flow Cytometry Specialist - Immunology

The mission of the Allen Institute is to unlock the complexities of bioscience and advance our knowledge to improve human health. Using an open science, multi-scale, team-oriented approach, the Allen Institute focuses on accelerating foundational research, developing standards and models, and cultivating new ideas to make a broad, transformational impact on science.

The mission of the Allen Institute for Immunology is to advance the fundamental understanding of human immunology through the study of immune health and diseases where excessive or impaired immune responses drive pathological processes. The Institute will employ a multi-disciplinary team approach in collaboration with academic centers of human immunology to generate novel mechanistic insights into the immune synapse in health and in diseases such as autoimmunity or oncology. The Institute will simultaneously provide a foundational data set and tools for future immunological research as well as a novel collaboration portal for the broader scientific community.

The High Resolution Flow Cytometry Specialist will be part of a multi-disciplinary team and will be responsible for performing core flow cytometry for the Allen Institute in a standardized, high throughput manner. This High Resolution Flow Cytometry Specialist will support delivery of high-quality flow cytometry and proteomics data sets and oversee quality control and data processing for the team. The High Resolution Flow Cytometry Specialist will provide guidance on panel design and cell sorting strategies to the team, as well as participate in the general operation and maintenance of the laboratory. The High Resolution Flow Cytometry Specialist is expected to determine methods and procedures on new assignments, write and contribute to new protocols and may oversee the activities of others in junior positions. Opportunities exist for the exceptional candidate to actively contribute to the analysis and interpretation of data from our longitudinal healthy, autoimmune, and cancer cohorts and expand our use of spectral cytometry and cell sorting. Good judgment and problem solving skills are required for recognizing process deviations, participating in troubleshooting, and operation of complex laboratory instrumentation. A successful candidate possesses excellent attention to detail and the eagerness to work in a Team Science, deadline-driven atmosphere.

The Allen Institute believes that team science significantly benefits from the participation of diverse voices, experiences, and backgrounds. High-quality science can only be produced when it includes different perspectives. We are committed to increasing diversity across every team and encourage people from all backgrounds to apply for this role.

Essential Functions

  • Perform flow cytometry techniques (cell sorting, detection of biomarkers and cell phenotyping)
  • Oversee flow data quality control and processing for the team
  • Provide technical support for panel design and optimization to other scientists
  • Develop protocols for assay validation and optimization, SOP, and scientific manuscript authoring
  • Support discovery projects as part of the Experimental Immunology core team
  • Meet experimental and data production schedules
  • Keep excellent experimental records of experiments
  • Follow standard operation procedures and good laboratory practices

Note: Reasonable accommodations may be made to enable individuals with disabilities to perform the essential functions. This description reflects managementís assignment of essential functions; it does not prescribe or restrict the tasks that may be assigned.

Required Education and Experience

  • Bachelorís degree in a life science
  • 4 years of relevant post-undergraduate research experience
  • Background in immunology; working knowledge of cell types and functional assays
  • Experience with analysis and sorting on a flow cytometer
  • Working knowledge of flow cytometry automation
  • Practical experience with flow cytometers and data analysis using FlowJo or equivalent
  • Attention to detail and strong problem-solving skills

Preferred Education and Experience

  • Masterís degree in a life science
  • 4-8 years of relevant post-undergraduate research experience
  • Experience with high parameter cytometers such as Cytek Aurora, BD FACSymphony A5, and BD FACSAria Fusion
  • Familiarity with spectral flow cytometry
  • Working knowledge of data QC and dimensionality reduction programs applied to flow cytometry
  • Basic programming skills and familiarity with Python and R
  • Experience with Mesoscale Diagnostics (MSD) proteomics immunoassays
  • Strong organizational and communication skills
  • Experience with a LIMS system
  • 1+ year experience working in a GLP or CLIA regulated environment

Work Environment

  • Open office seating
  • Bench top work in a BSL-2 laboratory environment, including potential exposure to biohazards

Physical Demands

  • Occasional lifting to 30 pounds
  • Requires repetitive multi-channel pipetting, single channel pipetting, and computer data entry
  • Fine motor movements in fingers/hands to operate computers and other office and lab equipment, repetitive motion with lab equipment
  • Stooping, bending, crouching
  • Reaching, or climbing ladders

Position Type/Expected Hours of Work

  • May need to work outside of standard working hours at times
  • This role is currently working onsite and is expected to work onsite for the majority of working hours. The primary work location for this role is 615 Westlake Ave N., with flexibility to work remotely on a limited basis.


  • Attendance and participation in national and international conferences encouraged

Additional Eligibility Qualifications

  • In keeping with our focus on employee safety, all employees must be fully vaccinated against COVID-19 as a condition of employment unless a medical or religious exemption is approved. All employees will be required to keep their vaccination status up to date according to CDC guidance and our policy.

Additional Comments

  • **Please note, this opportunity offers relocation assistance**
  • **Please note, this opportunity does not offer work visa sponsorship**

It is the policy of the Allen Institute to provide equal employment opportunity (EEO) to all persons regardless of age, color, national origin, citizenship status, physical or mental disability, race, religion, creed, gender, sex, sexual orientation, gender identity and/or expression, genetic information, marital status, status with regard to public assistance, veteran status, or any other characteristic protected by federal, state or local law. In addition, the Allen Institute will provide reasonable accommodations for qualified individuals with disabilities.





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