Careers at Allen Institute

Are you ready for new challenges and new opportunities?

Join our team!

Current job opportunities are posted here as they become available.

Neuroanatomy Intern - Investigating the synergism of amyloid beta and tau pathology in Alzheimerís disease mouse models

Department: 2360- Neuroanatomy
Location: Seattle

Title:

Investigating the synergism of amyloid beta and tau pathology in Alzheimerís disease mouse models

Project Overview:

Alzheimerís disease is a progressive, neurodegenerative disorder characterized by aggregation of misfolded beta amyloid and tau proteins. These two proteins have a complex pattern of interaction and can exacerbate each otherís effects. We have mapped whole-brain amyloid beta plaque distribution in Alzheimerís disease mouse models overexpressing mutant amyloid precursor protein (APP, https://bit.ly/2Go80tl) and are in the process of mapping brain wide axonal projections to examine changes in long-range connectivity during disease progression in APP-overexpressing mice. To determine the effect of pathological tau on whole brain amyloid beta pathology and axonal connectivity, we crossed one APP-overexpressing mouse line with another expressing a mutant form of tau. We are measuring whole-brain plaque distribution, changes in brain size, behavior, and axonal projection patterns in the offspring from this cross to quantify the additional pathology resulting from the combination of APP and tau expression. The goal of this project is to assist in the quantification of pathology by 1. Immunostaining mouse hippocampal and cortical brain sections for synaptic marker proteins, and 2. Quantifying synaptic density, tissue atrophy, and neurite dystrophy from image data. This project will involve performing experiments in the laboratory, acquisition of image data using widefield and confocal microscopy, and image analysis using Fiji.

Educational Objectives:

  • Learn about the mechanisms of Alzheimerís disease progression, including beta amyloid and tau pathologies and the correlation between amyloid pathologies and neurodegeneration.
  • Obtain general knowledge about neuroanatomy, rodent stereotaxic surgery, and serial two-photon imaging, although the surgeries and imaging for this project are already completed.
  • Learn skills related to immunohistochemistry and antibody-antigen interactions.
  • Practice experimental design techniques including hypothesis generation, power calculations, and choosing appropriate control experiments.
  • Develop technical skills for antibody labeling and mounting brain sections on slides.
  • Obtain knowledge and skills related to fluorescence microscopy including choosing fluorophores, widefield and confocal imaging, identifying signal and background, and multi-color imaging.
  • Learn methods for quantification, data analysis, and statistics in experimental datasets.
  • Produce figures and presentations.
  • Perform literature searches and critical reading of manuscripts.

Eligibility

  • U.S. Citizen or U.S. Permanent Resident currently enrolled in an accredited U.S. college or university.
  • Enrolled in undergraduate studies or in a Masterís program. You must be enrolled and returning to school Fall quarter.
  • Must be able to start on either June 3rd or June 17th, 2019 and commit to the full 10-12 week internship time period. Interns will be expected to work 35-40 hours a week.
  • Must be 18 years of age or older.

Compensation

Interns will receive $15.00 per hour which is equivalent to $6,000 less taxes.

Housing

Housing is not provided as a part of the internship program. If selected we will provide a list of housing resources for you.

Transportation

Interns will be provided with an ORCA business passport free of charge for the duration of their internship.

How to Apply

  1. Complete an application through this portal. The application deadline is January 30, 2019.
  2. Submit a resume as well as the following documents using the + supplemental documents button:
    • A personal statement describing your interest in Allen Institute for Brain Science
    • College transcript with Cumulative GPA and Courses taken (can be unofficial)

3. Send one letter of recommendation to HR@alleninstitute.org.

Contact us

If you have any further questions please contact us at HR@alleninstitute.org.

Applicant Tracking System Powered by ClearCompany HRM Applicant Tracking System