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Viral Technology Intern - light-inducible recombinase systems

Department: 2300-Structured Science
Location: Seattle

Title: Generation of light-inducible recombinase systems for genomic modification

Project Overview: Neural projections between brain areas comprise multiple cell types, each with distinct transcriptional, morphological, and physiological properties. Using recombinase-based tools to characterize the properties of individual cells within a specific projection requires precise spatiotemporal control of the recombinase system, such that single cells with potentially similar features may be differentially targeted. We have developed light-inducible recombinase systems to enable spatiotemporal targeting of genomic modification in single cells. The main goal for a 2019 research internship is to test the feasibility of one recombinase system for in vivo imaging and neuroanatomy. The intern will use transgenic or wild type mice expressing light-inducible recombinases to test in vivo imaging parameters, use available computational toolkits to analyze physiological responses in cortical neurons, and reconstruct neuronal morphologies using 3D reconstruction software. Results from this project will complement ongoing experiments using other recombinase systems within the Viral Technology group.††

Educational Objectives:

  • Gain knowledge about genomic modification techniques
  • Gain knowledge about visual coding and optical physiology
  • Gain knowledge about neuroanatomy and cell type specificity
  • Learn basic animal handling and surgical methods
  • Learn in vitro and in vivo fluorescence imaging methods
  • Learn basic coding skills and computational analysis


  • U.S. Citizen or U.S. Permanent Resident currently enrolled in an accredited U.S. college or university.
  • Enrolled in undergraduate studies or in a Masterís program. You must be enrolled and returning to school Fall quarter.
  • Must be able to start on either June 3rd or June 17th, 2019 and commit to the full 10-12 week internship time period. Interns will be expected to work 35-40 hours a week.
  • Must be 18 years of age or older.


Interns will receive $15.00 per hour which is equivalent to $6,000 less taxes.


Housing is not provided as a part of the internship program. If selected we will provide a list of housing resources for you.


Interns will be provided with an ORCA business passport free of charge for the duration of their internship.

How to Apply

  1. Complete an application through this portal. The application deadline is January 30, 2019.
  2. Submit a resume as well as the following documents using the + supplemental documents button:
    • A personal statement describing your interest in Allen Institute for Brain Science
    • College transcript with Cumulative GPA and Courses taken (can be unofficial)

3. Send one letter of recommendation to

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