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Molecular Genetics Intern - high-precision genetic tools

Department: 2390- Molecular Genetics
Location: Seattle

Title:

Generation of high-precision genetic tools for analysis of cell types within the mammalian brain

Project Overview:

The mammalian brain contains a rich diversity of cell types that function in a highly organized and coordinated manner to influence behavior. Recent efforts to catalog neuronal cell types based on their transcriptional profiles within the mouse neocortex have revealed entirely new classes of cell types. In order to systematically profile their properties at a single cell level and probe their activity within a neural circuit in an awake behaving animal we need to create new genetic tools. We aim to generate a large and diverse set of transgenic mouse lines and viruses for cell-type-specific investigations by building upon on our existing transgenic expression platforms and identifying enhancer elements from single cell epigenomics data. The main goal for the research internship will be to build and test a new genetic tool for labeling one cell type, either directly or via an intersectional genetic approach. The intern will design and construct a DNA vector using molecular cloning techniques and test it in vitro using a mammalian cell line. Transgene expression levels and subcellular localization will be determined by epifluorescence imaging and/or FACS analyses. Functionality of this tool or a similar one being generated within our production pipeline will also be analyzed in the mouse brain via standard histological methods to determine specificity and tractability.

Educational Objectives:

  • Gain knowledge about genome engineering techniques and viral transduction methods
  • Gain knowledge about mouse genetics
  • Gain knowledge about cutting edge tools in neuroscience
  • Learn basic neuroanatomy and histological methods
  • Learn basic molecular cloning techniques for vector generation
  • Learn basic cell culture techniques
  • Learn epifluorescence imaging
  • Gain experience working with an interdisciplinary group of scientists

Eligibility

  • U.S. Citizen or U.S. Permanent Resident currently enrolled in an accredited U.S. college or university.
  • Enrolled in undergraduate studies or in a Masterís program. You must be enrolled and returning to school Fall quarter.
  • Must be able to start on either June 3rd or June 17th, 2019 and commit to the full 10-12 week internship time period. Interns will be expected to work 35-40 hours a week.
  • Must be 18 years of age or older.

Compensation

Interns will receive $15.00 per hour which is equivalent to $6,000 less taxes.

Housing

Housing is not provided as a part of the internship program. If selected we will provide a list of housing resources for you.

Transportation

Interns will be provided with an ORCA business passport free of charge for the duration of their internship.

How to Apply

  1. Complete an application through this portal. The application deadline is January 30, 2019.
  2. Submit a resume as well as the following documents using the + supplemental documents button:
    • A personal statement describing your interest in Allen Institute for Brain Science
    • College transcript with Cumulative GPA and Courses taken (can be unofficial)

3. Send one letter of recommendation to HR@alleninstitute.org.

Contact us

If you have any further questions please contact us at HR@alleninstitute.org.

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