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Research Associate II - Imaging

Department: 2300-Structured Science
Location: Seattle

Research Associate II – Imaging

Our mission at the Allen Institute for Brain Science is to accelerate the understanding of how the human brain works in health and disease. Using a big science approach, we generate useful public resources, drive technological and analytical advances, and discover fundamental brain properties through integration of experiments, modeling and theory.

The Imaging department RA II, will assist with sample prep for spatial transcriptomics pipeline experiments performed on tissue sections derived from physiology and morphology experiments. This position will involve preparation of solutions for tissue fixation and clearing, DNA probe hybridization and amplification of fluorescent signal in preparation for imaging via light sheet/diSPIM microscopy as well as confocal microscopy. Attention to detail will be critical. This individual will join a team focused on identification of transcriptomic cell types in the context of intact human and mouse brain tissue.

JOB RESPONSIBILTIES

  • Molecular biology and bioinformatics
  • Immunohistochemistry and fluorescence microscopy
  • Ability to maintain and work in RNAse-free conditions
  • Working knowledge of diSPIM and confocal microscopy techniques
  • Effective communication of techniques and scientific results
  • Proficiency with the Microsoft Office suite, including Word, Excel and Powerpoint. This position also requires the ability to process and present images using image processing software such as Photoshop.
  • This position will involve a considerable amount of troubleshooting and independent work. The candidate must have the analytical capacity to determine the best course for improving the technique when needed.

REQUIED QUALIFICATIONS

  • Bachelor’s degree (B.A. or B.S.) from four-year college or university
  • Ability to read, analyze, and interpret technical procedures/reports/documents and SOPS, to clearly describe the procedure used.
  • 2-4 years related experience should be in the areas of molecular biology, transcriptomics, histology, microscopy, or biochemistry.

PREFERRED QUALIFICATIONS and EXPERIENCE

  • The ideal candidate should be able to assess technical advances in primary literature.
  • Proficiency in bioinformatics tools for the purposes of DNA probe design.

???????WORK ENVIRONMENT

  • Sitting, standing, bending, squatting as found in typical office environment.
  • Occasional exposure to laboratory atmosphere - possible exposure to chemical, biological or other hazardous substances.
  • This position requires the manual dexterity sufficient to operate pipettes, phones, computers, and other office equipment.

 

 

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